DETECTION OF MUTATIONS IN AVIAN REOVIRUS dsRNA GENOME BY DENATURING GRADIENT (DGGE) AND CONSTANT DENATURING (CDGE) GEL ELECTROPHORESIS

Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis (DGGE) in combination with PCR and 'GC-clamping' has proven highly efficient as a method for detection of DNA sequence differences. Due to strand dissociation phenomena, however, its use has been limited to the analysis of sequences with a relatively low content of GC pairs. This paper describes how treatment of template DNA with sodium bisulphite drastic...

Microbial Community Fingerprints by Differential Display Denaturing Gradient 1 Gel Electrophoresis ( DD - DGGE )

Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology – Insights from Freshwaters

Although freshwater ecosystems support a great diversity of life, knowledge of its total diversity is far to be complete, particularly among microbes (Dudgeon et al., 2006). Aquatic microorganisms are pivotal in several biogeochemical processes, playing a key role in the recycling of organic material, or contributing to energy transfer in food webs, since they constitute food sources to micro-f...

Rapid detection of avian Eimeria species using denaturing gradient gel electrophoresis.

A denaturing gradient gel electrophoresis (DGGE) assay was developed to rapidly discriminate species of avian Eimeria. Amplification by PCR of the small subunit ribosomal RNA gene (approximately 1,600 nucleotides) with Eimeria genus-specific primers followed by cloning and sequencing allowed us to carry out phylogenetic analyses and identify clone sequences to species level in most cases. Clone...

Application of denaturing gradient gel electrophoresis (DGGE) for assessing fecal pollution sources at a recreational beach.

We investigated the effectiveness of Escherichia coli community fingerprinting for identifying fecal pollution sources impacting a recreational beach. E. coli in water collected from the beach, nearby creek and storm sewer outfall were enumerated using membrane filtration, while E. coli communities were characterized following polymerase chain reaction analysis and denaturing gradient gel elect...